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Journal / Immunology and Infection / Morfologisk og sammensætningsanalyse af…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Morfologisk og sammensætningsanalyse af neutrofile ekstracellulære fælder induceret af mikrobielle og kemiske stimuli
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Morfologisk og sammensætningsanalyse af neutrofile ekstracellulære fælder induceret af mikrobielle og kemiske stimuli

Article DOI: 10.3791/64522-v • 14:05 min • November 4th, 2022
November 4th, 2022 •
1, 2, 1, 3, 4, 3, 1
1Clinical Immunology Research Department of Biochemical Sciences Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 2Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 3CONACYT- Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 4Molecular Immunology Research Department of Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca

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Her præsenteres en protokol til induktion og analyse af in vitro neutrofile ekstracellulære fælder (NET'er). Kvantificering af DNA, cathelicidin (LL37) og enzymaktivitet gav data, der viser variabiliteten i sammensætningen og morfologien af NET'er induceret af mikrobielle og kemiske stimuli under lignende kontrollerede forhold.

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Immunologi og infektion nummer 189
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