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Biochemistry

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JoVE Journal Biochemistry
Concanavaline A-gebaseerde sedimentatietest om substraatbinding van glucaanfosfatasen te meten
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Concanavaline A-gebaseerde sedimentatietest om substraatbinding van glucaanfosfatasen te meten

Article DOI: 10.3791/64700-v • 09:07 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1
1Department of Chemistry, Skidmore College
* These authors contributed equally

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Deze methode beschrijft een op lectine gebaseerde in vitro sedimentatietest om de bindingsaffiniteit van glucaanfosfatase en amylopectine te kwantificeren. Deze co-sedimentatietest is betrouwbaar voor het meten van glucaanfosfatasesubstraatbinding en kan worden toegepast op verschillende opgeloste glucaansubstraten.

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