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Biochemistry

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JoVE Journal Biochemistry
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
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Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Article DOI: 10.3791/64247-v • 06:02 min • August 23rd, 2022
August 23rd, 2022 •
1, 1, 1
1Aptagen LLC

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Summary
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The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.

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Thermodynamic Kinetic Association DNA Aptamer Tetracycline Isothermal Titration Calorimetry Binding Affinities Mechanism Of Binding ITC Labeled Functionalized SPR Buffers Sample Requirements Proteins Dialysis Column PBS Buffer Centrifuge Microliters Pipette UV-visible Spectrometer Concentration
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