Journal
/
Biochemistry
/
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

DOI:
10.3791/64247-v

•

06:02 min

•

August 23, 2022

•

, ,
1Aptagen LLC

Chapters

  • 00:04Introduction
  • 00:40Preparation of Samples
  • 02:23Running the Test Kit and the Sample to Determine the Binding Between Aptamer and Tetracycline
  • 03:26Analyzing Data Using Software
  • 04:22Results: ITC Thermogram of a DNA Aptamer and Tetracycline
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.

Tags

Keywords: DNA AptamerTetracyclineIsothermal Titration Calorimetry (ITC)Binding AffinityThermodynamic And Kinetic AssociationBuffer ExchangeAptamer FoldingDegassingITC Measurement

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64247?article_type=v

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
check_url/64247?article_type=v

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
check_url/64247?article_type=v

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
check_url/64247?article_type=v

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
check_url/64247?article_type=v

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
check_url/64247?article_type=v

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
check_url/64247?article_type=v

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
check_url/64247?article_type=v

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
check_url/64247?article_type=v

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
check_url/64247?article_type=v

Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules

Read Article