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Journal / Neuroscience / Intravital billeddannelse af fluorescerende…
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Neuroscience

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JoVE Journal Neuroscience
Intravital billeddannelse af fluorescerende proteinekspression hos mus med en lukket kranium traumatisk hjerneskade og kranievindue ved hjælp af et to-fotonmikroskop
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Intravital billeddannelse af fluorescerende proteinekspression hos mus med en lukket kranium traumatisk hjerneskade og kranievindue ved hjælp af et to-fotonmikroskop

Article DOI: 10.3791/64701-v • 08:25 min • April 21st, 2023
April 21st, 2023 •
1,2, 3, 1, 3, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology, University of Massachusetts Chan Medical School

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Denne undersøgelse viser levering af en gentagen traumatisk hjerneskade på mus og samtidig implantation af et kranievindue til efterfølgende intravital billeddannelse af en neuron-udtrykt EGFP ved hjælp af to-fotonmikroskopi.

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Intravital billeddannelse fluorescerende proteinekspression mus lukket kranium traumatisk hjerneskade kranievindue to-fotonmikroskop protein af interesse eksogene stimuli intrakraniel injektion adeno-associeret virus (AAV) forbedret grønt fluorescerende protein (EGFP) neuronal specifik promotor vægttabsenhed metalhovedstolpe glaskranivindue ekspression og cellulær lokalisering langsgående visualisering
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