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Journal / Neuroscience / 使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像…
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Neuroscience

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JoVE Journal Neuroscience
使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像
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使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

Article DOI: 10.3791/64701-v • 08:25 min • April 21st, 2023
April 21st, 2023 •
1,2, 3, 1, 3, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology, University of Massachusetts Chan Medical School

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这项研究表明，向小鼠提供重复性创伤性脑损伤，并同时植入颅窗，以便随后使用双光子显微镜对神经元表达的 EGFP 进行活体成像。

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活体成像、荧光蛋白表达、小鼠、闭合性颅骨创伤性脑损伤、颅窗、双光子显微镜、目标蛋白、外源性刺激、颅内注射、腺相关病毒 （AAV）、增强型绿色荧光蛋白 （EGFP）、神经元特异性启动子、减重装置、金属头柱、玻璃颅窗、表达和细胞定位、纵向可视化
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