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Journal / Immunology and Infection / Gentechnisch veränderte Plasmodium berghei…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Gentechnisch veränderte Plasmodium berghei Sporozoiten
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Gentechnisch veränderte Plasmodium berghei Sporozoiten

Article DOI: 10.3791/64992-v • 10:16 min • May 5th, 2023
May 5th, 2023 •
1, 1,2
1Center for Tropical and Emerging Global Diseases, University of Georgia, 2Department of Cellular Biology, University of Georgia

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Malaria wird durch Inokulation des Sporozoitenstadiums von Plasmodium durch infizierte Stechmücken übertragen. Transgenes Plasmodium hat es uns ermöglicht, die Biologie der Malaria besser zu verstehen und hat direkt zur Entwicklung von Malaria-Impfstoffen beigetragen. In dieser Arbeit beschreiben wir eine optimierte Methodik zur Generierung transgener Plasmodium berghei-Sporozoite.

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Genetisch veränderte Plasmodium berghei Sporozoiten Malaria Parasiten-Plasmodium Anopheles-Mücken Entwicklung im Leberstadium klinische Malaria Immunantwort transgene Parasiten Blutstadium P. berghei Stechmückenspeicheldrüsen In-vivo-Experimente In-vitro-Experimente Grün fluoreszierendes Protein (GFP) Malaria im Leberstadium
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