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A Simple Technique to Assay Locomotor Activity in Drosophila
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JoVE Journal Behavior
A Simple Technique to Assay Locomotor Activity in Drosophila

A Simple Technique to Assay Locomotor Activity in Drosophila

DOI:
10.3791/65092-v

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07:47 min

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February 24, 2023

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, , ,
1Institute of Neuroscience,Soochow University, 2Department of Neurology and Clinical Research Center of Neurological Disease,The Second Affiliated Hospital of Soochow University

Chapters

  • 00:05Introduction
  • 00:58Experimental Preparation
  • 01:53Video Recording and Preprocessing
  • 02:41Video Analysis
  • 04:13Tracking File Analysis
  • 04:58Analysis per Frame
  • 05:41Results: Evaluation of Locomotor Activity in Adult and Individual Larval Flies
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol assesses the locomotor activity of Drosophila by tracking and analyzing the movement of flies in a hand-made arena using open-source software Fiji, compatible with plugins to segment pixels of each frame based on high-definition video recording to calculate parameters of speed, distance, etc.

Tags

Keywords: DrosophilaLocomotor ActivityOpen-source SoftwareVideo TrackingBehavioral AnalysisSilica ArenaCamera SetupFiji SoftwareVideo ConversionTracking Parameters

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