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Journal / Immunology and Infection / Verrijking van inheemse en recombinante…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Verrijking van inheemse en recombinante extracellulaire blaasjes van mycobacteriën
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Verrijking van inheemse en recombinante extracellulaire blaasjes van mycobacteriën

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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Dit protocol beschrijft de verrijking van inheemse mycobacteriële extracellulaire vesikels (mEV's) uit axenische culturen van Mycobacterium smegmatis (Msm) en hoe mCherry (een rode fluorescerende reporter)-bevattende recombinante MsmEV's kunnen worden ontworpen en verrijkt. Ten slotte verifieert het de nieuwe aanpak met de verrijking van MsmEV's die het EsxA-eiwit van Mycobacterium tuberculosis bevatten.

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Immunologie en infectie Extracellulaire blaasjes recombinante blaasjes Esat-6 mycobacteriën ExsA mCherry Cfp-29 bacteriën
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