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Journal / Immunology and Infection / Mikobakterilerin Doğal ve Rekombinant Hücre Dışı…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Mikobakterilerin Doğal ve Rekombinant Hücre Dışı Veziküllerinin Zenginleştirilmesi
Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes
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Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
Journal (Immunology and Infection)
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Mikobakterilerin Doğal ve Rekombinant Hücre Dışı Veziküllerinin Zenginleştirilmesi

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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Bu protokol, Mycobacterium smegmatis'in (Msm) aksenik kültürlerinden doğal mikobakteriyel hücre dışı veziküllerin (mEV'ler) zenginleştirilmesini ve mCherry (kırmızı floresan raportör) içeren rekombinant MsmEV'lerin nasıl tasarlanabileceğini ve zenginleştirilebileceğini detaylandırır. Son olarak, Mycobacterium tuberculosis'in EsxA proteinini içeren MsmEV'lerin zenginleştirilmesiyle yeni yaklaşımı doğrulamaktadır.

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İmmünoloji ve Enfeksiyon Sayı 202 Hücre dışı veziküller rekombinant veziküller Esat-6 mikobakteriler ExsA mCherry Cfp-29 bakteriler
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