Imaging Vital and Non-vital Brain Pericytes in Brain Slices following Subarachnoid Hemorrhage

Yong-Jin Zhang; Yun-Cong Li; Han-Fu Yu; Chong Li; Hong-Ji Deng; Yue-Hong Dong; Gui-Bo Li; Fei Wang

doi:10.3791/65873

Journal

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Neuroscience

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Imaging Vital and Non-vital Brain Pericytes in Brain Slices following Subarachnoid Hemorrhage

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Imaging Vital and Non-vital Brain Pericytes in Brain Slices following Subarachnoid Hemorrhage

1,005 Views

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05:11 min

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August 18, 2023

DOI:

10.3791/65873-v

DOI:

10.3791/65873-v

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05:11 min

August 18, 2023

•

7 Views

Yong-Jin Zhang^1,2, Yun-Cong Li¹, Han-Fu Yu¹, Chong Li¹, Hong-Ji Deng¹, Yue-Hong Dong², Gui-Bo Li¹, Fei Wang¹

¹Department of Neurosurgery,The First Affiliated Hospital of Kunming Medical University, ²Clinical Medical Research Center,The First Affiliated Hospital of Kunming Medical University

Transcript

Our study showed a significant increase in pericytes deaths. So starting at a sick horse after SAH, it demonstrated a positive correlation between the number of low vital parasites and the time elapsed after SAH. Imaging vital and the non-vital brain parasites in brain slices following SAH and the parasite isolation techniques, have advanced the research in my field.

Currently, it’s quite difficult to fully distinguish between dead parasites and dead endothelial cells. Our study find contraction-dependent apoptosis in parasites after SAH. We have developed a very reliable protocol, which enables the fluorescent labeling, both functional and a non-functional brain parasite in brain sections, and the same time.

Summary

The preliminary inquiry confirms that subarachnoid hemorrhage (SAH) causes brain pericyte demise. Evaluating pericyte contractility post-SAH requires differentiation between viable and non-viable brain pericytes. Hence, a procedure has been developed to label viable and non-viable brain pericytes concurrently in brain sections, facilitating observation using a high-resolution confocal microscope.