Measuring Bacterial Colonization on <i>Arabidopsis thaliana</i> Roots in Hydroponic Condition

Xia Shu; Huatai Li; Jing Wang; Shan Wang; Yunpeng Liu; Ruifu Zhang

doi:10.3791/66241

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Measuring Bacterial Colonization on Arabidopsis thaliana Roots in Hydroponic Condition

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Measuring Bacterial Colonization on Arabidopsis thaliana Roots in Hydroponic Condition

Measuring Bacterial Colonization on Arabidopsis thaliana Roots in Hydroponic Condition

626 Views

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05:37 min

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March 01, 2024

DOI:

10.3791/66241-v

DOI:

10.3791/66241-v

•

05:37 min

March 01, 2024

•

13 Views

Xia Shu^1,2, Huatai Li¹, Jing Wang³, Shan Wang⁴, Yunpeng Liu¹, Ruifu Zhang^1,3

¹State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences, ²State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology,Huazhong Agricultural University, ³Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University, ⁴Jiangsu Engineering and Technology Center for Modern Horticulture,Jiangsu Vocational College of Agriculture and Forestry

Transcript

Our research focuses on the interaction of beneficial rhizobacteria with plant roots. We are looking for the key mechanisms to enhance the colonization of beneficial bacteria, thus improving the efficiency and the stability of microbial fertilizers in the field. The methods for bacterial colonization of plant roots have been extensively studied, but there are still problems.

For example, it’s hard to measure the colonization on different sites distinctively, In the future we think applying microscope and high throughput counting will help to solve the problems. Our protocol is highly applicable with good repeatability, and it is more suitable for accurate rhizosphere bacteria colonization determination.

Summary

Colonization of plant growth-promoting rhizobacteria (PGPR) in the rhizosphere is essential for its growth-promoting effect. It is necessary to standardize the method of detection of bacterial rhizosphere colonization. Here, we describe a reproducible method for quantifying bacterial colonization on the root surface.