JoVE Journal
Biology
Biology
This content is Free Access.
Chapters
Summary
Please note that all translations are automatically generated.
We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific markers.