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Fotoconversione di proteine ​​purificate fluorescenti ed evidenziazione ottica dual-sonda in cellule vive
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Fotoconversione di proteine ​​purificate fluorescenti ed evidenziazione ottica dual-sonda in cellule vive

Article DOI: 10.3791/1995-v • 11:22 min • June 26th, 2010
June 26th, 2010 •
1, 1
1Department of Molecular Physiology and Biophysics, Vanderbilt University

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Questo protocollo descrive un approccio generale per eseguire fotoconversione di proteine ​​fluorescenti su un microscopio confocale a scansione laser. Si descrivono le modalità per la fotoconversione di campioni di proteine ​​puried, nonché per evidenziare ottici a doppia sonda in cellule vive con mOrange2 e Dronpa.

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Biologia Cellulare Numero 40 Morange Dronpa fotoconversione fotoattivazione ottanolo gocciolina confocale imaging
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