JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
在活细胞荧光蛋白的双探头光学突出纯净的光转化的
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Please note that all translations are automatically generated.
Click here for the English version.
在活细胞荧光蛋白的双探头光学突出纯净的光转化的
DOI:
10.3791/1995-v
•
11:21 min
•
June 26, 2010
•
Gert-Jan Kremers
,
David Piston
1
Department of Molecular Physiology and Biophysics
,
Vanderbilt University
Chapters
00:03
Title
00:57
Introduction
01:26
Preparing Fluorescent Protein Droplet Samples
03:59
Setting up a Photoconversion Experiment
06:57
Dual-Probe Optical Highlighting
09:09
Representative Results
10:48
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这个协议描述的一般方法执行在激光共聚焦显微镜,荧光蛋白的光转化。我们描述puried蛋白质样品的光转化,以及与mOrange2和Dronpa活细胞的双探头光学突出的程序。
Tags
Photoconversion
Fluorescent Proteins
Optical Highlighter
Live Cells
Marking
Subpopulation
Tracking
Intensity
Exposure Time
Photoconversion Light
Photobleach
Confocal Laser Scanning Microscope
Purified Protein Droplet Samples
Photophysical Behavior
Microscope Configuration
Dual-probe Optical Highlighting
MOrange2
Dronpa
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
与非侵入性检测荧光染料标记的干细胞与光学成像
适当的照顾和清洁的显微镜
相衬,微分干涉对比(DIC)显微镜
对活细胞成像的SNAP - Tag融合蛋白的荧光标记的COS - 7
偏光易位荧光蛋白
爪蟾
外胚层响应Wnt信号
GFP标记的蛋白质在实时成像<em>果蝇</em>卵母细胞
活体显微镜成像的亚细胞结构的活体小鼠表达荧光蛋白
在
造血干细胞和祖细胞利用共聚焦和多光子显微镜五荧光蛋白标记的
活体
克隆跟踪
纯化野生型和突变CFTR蛋白的功能重建和通道活性测定
钙化事件的表征在海洋Tubeworm使用实时光学和电子显微镜技术
Read Article