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在活细胞荧光蛋白的双探头光学突出纯净的光转化的
JoVE Journal
Biology
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JoVE Journal Biology
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
DOI:

11:21 min

June 26, 2010

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Chapters

  • 00:03Title
  • 00:57Introduction
  • 01:26Preparing Fluorescent Protein Droplet Samples
  • 03:59Setting up a Photoconversion Experiment
  • 06:57Dual-Probe Optical Highlighting
  • 09:09Representative Results
  • 10:48Conclusion

Summary

Automatic Translation

这个协议描述的一般方法执行在激光共聚焦显微镜,荧光蛋白的光转化。我们描述puried蛋白质样品的光转化,以及与mOrange2和Dronpa活细胞的双探头光学突出的程序。

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