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Journal / Biology / Intravenös Microinjections av Zebrafish Larver…
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Intravenös Microinjections av Zebrafish Larver att studera akut njurskada
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Intravenös Microinjections av Zebrafish Larver att studera akut njurskada

Article DOI: 10.3791/2079-v • 15:56 min • August 4th, 2010
August 4th, 2010 •
1, 2, 3, 1
1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Department of Medicine and Genetics, Harvard Medical School

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Vi beskriver en teknik för microinjecting aminoglykosid, gentamicin, i 2 dagar efter fetilization (DPF) zebrafisk larver att inducera akut njurskada (AKI). Vi beskriver en metod för hela berget immunohistokemi, plast inbäddning och snittning av zebrafisk larver att visualisera AKI medierad skador.

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Utvecklingsbiologi intravenös mikroinjektion zebrafisk gentamicin akut njurskada
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