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FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
JoVE Journal
Biology
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JoVE Journal Biology
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
DOI:

10:34 min

August 20, 2012

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Chapters

  • 00:05Title
  • 01:38FRET Imaging Microscope Assembly
  • 03:34Imaging Software Installation and Configuration
  • 05:37Live-cell Imaging with FRET
  • 08:35Monitoring Intracellular cAMP by FRET Imaging
  • 09:42Conclusion

Summary

Automatic Translation

Förster resonance energy transfer (FRET) microscopy is a powerful technique for real-time monitoring of signaling events in live cells using various biosensors as reporters. Here we describe how to build a customized epifluorescence FRET imaging system from commercially available components and how to use it for FRET experiments.

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