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JoVE Journal Neuroscience
Imaging Analyse av Neuron til gliaceller samhandling i Microfluidic Kultur Platform (MCP)-baserte Neuronal Axon og gliaceller Co-kultur System
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Imaging Analyse av Neuron til gliaceller samhandling i Microfluidic Kultur Platform (MCP)-baserte Neuronal Axon og gliaceller Co-kultur System

Article DOI: 10.3791/4448-v • 09:34 min • October 14th, 2012
October 14th, 2012 •
1, 1,2
1Department of Neuroscience, Tufts University, 2Neuroscience Program, Tufts Sackler School of Graduate Biomedical Sciences

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Denne studien beskriver prosedyrene for å sette opp en ny neuronal axon og (astro) gliaceller co-kultur plattform. I dette samarbeidet kultur systemet, blir manipulering av direkte interaksjon mellom en enkelt axon (og eneste glial celle) gjennomførbart, slik mekanistisk analyse av gjensidig nervecelle til glial signalering.

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Nevrovitenskap Molecular Biology cellebiologi biofysikk MicroFluidics Microfluidic kultur plattform compartmented kultur Neuron til gliaceller signalering nevroner gliaceller cellekultur
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