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JoVE Journal Medicine
Microarray-baserade Identifiering av individuella HERV Loci Expression
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Microarray-baserade Identifiering av individuella HERV Loci Expression: Anmälan till biomarkörer vid prostatacancer

Article DOI: 10.3791/50713-v • 13:19 min • November 2nd, 2013
November 2nd, 2013 •
1,2, 1,2, 1,3,4, 1,2, 5, 1,4,6, 1,4,7, 1,2
1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department, BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 4Medical Faculty, Lyon 1 University, 5Data and Knowledge Laboratory, BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon

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Mänskliga endogena retrovirus (HERV), som upptar 8% av det mänskliga genomet, behålla knappa kodning kapacitet men hundratusen långa terminala upprepningar (LTR). En anpassad Affymetrix microarray var utformad för att identifiera enskilda HERV locus uttryck och användes på prostatacancervävnad som ett proof of concept för framtida kliniska studier.

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Medicin Cancerbiologi genetik molekylärbiologi Prostata Retroviridae biomarkörer Farmakologisk tumörmarkörer biologiska prostatektomi microarray analys genuttryck diagnos Human Endogenous Retroviruses HERV microarray Transcriptome prostatacancer Affymetrix
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