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Neuroscience

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Brain Slice Biotinylering
 
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Brain Slice Biotinylering: En

Article DOI: 10.3791/51240-v 06:18 min April 3rd, 2014
April 3rd, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

Neuronal membran menneskehandel dynamisk styrer plasmamembranprotein tilgængelighed og markant indvirkning neurotransmission. Til dato har det været en udfordring at måle neuronal endocytisk handel med voksne neuroner. Her beskriver vi en meget effektiv, kvantitativ metode til måling af hurtige ændringer i overfladeprotein ekspression ex vivo i akutte hjernesnit.

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Neuroscience menneskehandel endocytose internalisering biotinylering hjerne neuroner transportør protein kinase C
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