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JoVE Journal Neuroscience
Super-opløsning Imaging af neuronale Dense-core blærer
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Super-opløsning Imaging af neuronale Dense-core blærer

Article DOI: 10.3791/51394-v • 09:30 min • July 2nd, 2014
July 2nd, 2014 •
1,2, 2, 3, 2,4
1Department of Physics, Lewis & Clark College, 2Program in Biochemistry and Molecular Biology, Lewis & Clark College, 3Jungers Center for Neuroscience Research, Oregon Health & Science University, 4Department of Chemistry, Lewis & Clark College

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Vi beskriver, hvordan man gennemfører fotoaktiveret lokalisering mikroskopi (PALM)-baserede studier af vesikler i faste, dyrkede neuroner. De centrale elementer i vores protokol omfatter mærkning blærer med photoconvertible kimærer, indsamling af spredte, indsamlede rå billeder med en super-opløsning mikroskopi system og behandling af RAW-billeder til at producere en super-opløsning.

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Neuroscience fotoaktiveret lokalisering mikroskopi Dendra2 tæt-core vesikel synapse hippocampus neuron klynge
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