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JoVE Journal Neuroscience
Super-upplösning avbildning av neurala Tät-core Blåsor
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Super-upplösning avbildning av neurala Tät-core Blåsor

Article DOI: 10.3791/51394-v • 09:30 min • July 2nd, 2014
July 2nd, 2014 •
1,2, 2, 3, 2,4
1Department of Physics, Lewis & Clark College, 2Program in Biochemistry and Molecular Biology, Lewis & Clark College, 3Jungers Center for Neuroscience Research, Oregon Health & Science University, 4Department of Chemistry, Lewis & Clark College

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Vi beskriver hur man ska genomföra photoactivated lokalisering mikroskopi (PALM)-baserade studier av blåsor i anläggningstillgångar, odlade nervceller. Viktiga komponenter i våra protokoll omfattar märknings blåsor med photoconvertible chimärer, samla råbilder glest samplade med en super-upplösning mikroskopi systemet, och bearbetar råbilder för att producera en super-upplösning.

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Neurovetenskap photoactivated lokalisering mikroskopi Dendra2 tät-core vesikler synaps hippocampus neuron kluster
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