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Journal / Biology / En kanal Cell-festet Patch-clamp opptak
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En kanal Cell-festet Patch-clamp opptak
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En kanal Cell-festet Patch-clamp opptak

Article DOI: 10.3791/51629-v • 13:07 min • June 9th, 2014
June 9th, 2014 •
1, 2, 1, 3, 4
1Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY, 2Department of Biochemistry, SMBS, University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department, The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY

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Beskrevet her er en prosedyre for å få lange strekninger av dagens opptak fra en ionekanal med celle-festet patch-clamp teknikken. Denne metoden gjør det mulig for å observere, i sanntid, mønsteret av open-nære kanal konformasjoner som ligger til grunn for biologisk signal. Disse dataene informere om kanal eiendommer i uforstyrrede biologiske membraner.

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Neuroscience biofysikk ionekanaler single-kanalopptak NMDA reseptorer gating elektrofysiologi patch-clamp kinetisk analyse
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