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JoVE Journal
Immunology and Infection

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A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
 

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Article DOI: 10.3791/51693-v 07:43 min June 5th, 2014
June 5th, 2014

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Summary

Microdissection has been extensively employed for the examination of DNA, RNA, and protein within tissue. Laser capture microscopy (LCM) is the most commonly used method, but a new milling technique, mesodissection, is recently available. We demonstrate RNA extraction from mesodissected formalin fixed paraffin embedded tissue slides of Mycobacterium tuberculosis granulomas.

Tags

Microdissection Mycobacterium Tuberculosis Formalin Fixed Paraffin Embedded Lung Granulomas Laser Capture Microscopy LCM Mesodissection MESO-1/MeSectr System FFPE Slide H&E Reference Slide
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