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Journal / Immunology and Infection / Analisi rapida di aberrazioni cromosomiche in…
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JoVE Journal Immunology and Infection
Analisi rapida di aberrazioni cromosomiche in linfociti B mouse da PNA-FISH
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Analisi rapida di aberrazioni cromosomiche in linfociti B mouse da PNA-FISH

Article DOI: 10.3791/51806-v • 07:54 min • August 19th, 2014
August 19th, 2014 •
1, 1
1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey

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Percorsi di riparazione del DNA sono essenziali per il mantenimento dell'integrità genomica e prevenire la mutazione e il cancro. L'obiettivo di questo protocollo è quello di quantificare l'instabilità genomica mediante l'osservazione diretta di aberrazioni cromosomiche in metafase si diffonde dalle cellule B del mouse utilizzando ibridazione in situ fluorescente (FISH) per ripetizioni telomeriche del DNA.

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Immunologia instabilità genomica la riparazione del DNA il mouse la diffusione metafase FISH coltura primaria
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