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Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
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Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

DOI:
10.3791/51806-v

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07:54 min

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August 19, 2014

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1Department of Molecular Biology and Biochemistry,Rutgers, the State University of New Jersey

Chapters

  • 00:05Title
  • 01:02B Cell Isolation and Activation
  • 03:08B Cell Fixation, Metaphase Chromosome Spread Preparation, and Telomere PNA FISH
  • 06:10Results: Chromosome Aberrations in Mouse B Cells as Visualized by PNA Telomere FISH
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

DNA repair pathways are essential for maintenance of genomic integrity and preventing mutation and cancer. The goal of this protocol is to quantify genomic instability by direct observation of chromosome aberrations in metaphase spreads from mouse B cells using fluorescent in situ hybridization (FISH) for telomeric DNA repeats.

Tags

Chromosome AberrationsMouse B LymphocytesPNA-FISHDNA RepairGenomic InstabilityTumorigenesisMetaphase Chromosome SpreadsPloidyMitotic IndexPrimary Cell PopulationTransformed FibroblastsPatient Cell Lines

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