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Journal / Bioengineering / Concentric Gel-System, um die biophysikalische…
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Bioengineering

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JoVE Journal Bioengineering
Concentric Gel-System, um die biophysikalische Rolle der Matrix Mikromilieu auf 3D-Zellmigration Studieren
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Concentric Gel-System, um die biophysikalische Rolle der Matrix Mikromilieu auf 3D-Zellmigration Studieren

Article DOI: 10.3791/52735-v • 11:43 min • April 3rd, 2015
April 3rd, 2015 •
1, 2, 2,3
1Systems Biophysics Department, FOM Institute AMOLF, 2Mechanobiology Institute, National University of Singapore, 3Department of Biomedical Engineering, National University of Singapore

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Die mechanischen Eigenschaften und die Mikrostruktur der extrazellulären Matrix stark beeinflussen 3D Migration von Zellen. In vitro-Verfahren, um die Raumzeit-Zellmigrationsverhalten in biophysikalisch variable Umgebungen sowohl Bevölkerung und einzelnen Zellebenen zu studieren, wird beschrieben.

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Bioengineering Zellmigration Kollagen Biomechanik 3D-Zellkultur Abbildung lebender Zellen Krebsinvasion Metastasierung extrazellulären Matrix Porengröße Biopolymer Zytoskelett konfokale Mikroskopie
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