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JoVE Journal
Developmental Biology

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使用非整合附加型质粒从冷冻血沉棕黄层诱导多能干细胞的产生
 
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使用非整合附加型质粒从冷冻血沉棕黄层诱导多能干细胞的产生

Article DOI: 10.3791/52885-v 10:52 min June 5th, 2015
June 5th, 2015

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Summary

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Click here for the English version.

诱导多能干细胞(iPS细胞)代表的患者特异性组织为临床应用和基础研究的来源。在这里,我们提出了一个详细的协议来重新编程从冷冻棕黄色大衣将获得使用非整合游离病毒质粒无iPSCs的人外周血单个核细胞(外周血单个核细胞)。

Tags

发育生物学,第100,干细胞生物学,细胞生物学,分子生物学,诱导多能干细胞,外周血单核细胞,重编程,附加型质粒。
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