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JoVE Journal Developmental Biology
Generatie van geïnduceerde pluripotente stamcellen van Frozen Buffy Coats behulp van niet-integrerende Episomaal plasmiden
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Generatie van geïnduceerde pluripotente stamcellen van Frozen Buffy Coats behulp van niet-integrerende Episomaal plasmiden

Article DOI: 10.3791/52885-v • 10:52 min • June 5th, 2015
June 5th, 2015 •
*1, *1, 1, 1, 2, 2, 3, 1, 1, 1
1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute
* These authors contributed equally

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Geïnduceerde pluripotente stamcellen (iPSCs) vormen een bron van patiënt-specifieke weefsels voor klinische toepassingen en fundamenteel onderzoek. Hier presenteren we een gedetailleerd protocol voor humane perifere bloed mononucleaire cellen (PBMNCs) die van bevroren buffy coats in viraal-vrije iPSCs met niet-integrerende episomale plasmiden herprogrammeren.

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Developmental Biology stamcelbiologie celbiologie moleculaire biologie geïnduceerde pluripotente stamcellen perifere mononucleaire bloedcellen herprogrammering episomale plasmiden.
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