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JoVE Journal
Biology

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使用荧光显微镜驱动蛋白-1的货物的识别
 
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使用荧光显微镜驱动蛋白-1的货物的识别

Article DOI: 10.3791/53632-v 08:06 min February 14th, 2016
February 14th, 2016

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

在这里,提出一个协议,以确定驱动蛋白-1的货物。该驱动蛋白1重链(KIF5B)的无电机突变体聚集在细胞质和诱导其货物的聚集。两个聚集体在荧光显微镜下检测。类似的策略可以用于识别其他马达蛋白的货物。

Tags

生物化学,第108,C-MYC,驱动蛋白1,KLC1,KIF5B,驱动蛋白,肌球蛋白,贩卖,显性负,荧光蛋白,荧光显微镜
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