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/
采用先进的3D荧光显微镜定量分析自噬
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
DOI:

09:59 min

May 03, 2013

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Chapters

  • 00:05Title
  • 01:27Preparing Live Cell Specimens for Imaging
  • 03:103D (Deconvolution) Fluorescence Microscopy
  • 06:10Results: Quantitative Image-Based Analysis of Cells Undergoing Autophagy
  • 09:02Conclusion

Summary

Automatic Translation

细胞自噬是一个无处不在的进程,使细胞蛋白质和细胞器的降解和回收利用。我们采用先进的荧光显微镜可视化和量化小,但必要的,与诱导自噬相关的物理变化,包括自噬体和溶酶体的形成和分布,以及他们融合成auto​​lysosomes。

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