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Journal
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Biology
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可视化单分子络合物
在体内
使用高级荧光显微
JoVE Journal
Biology
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JoVE Journal
Biology
Visualizing Single Molecular Complexes
In Vivo
Using Advanced Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
可视化单分子络合物
在体内
使用高级荧光显微
DOI:
10.3791/1508-v
•
11:26 min
•
September 08, 2009
•
Ian M. Dobbie
,
Alexander Robson
,
Nicolas Delalez
,
Mark C. Leake
1
Biochemistry
,
University of Oxford
,
2
Physics
,
University of Oxford
Chapters
00:04
Title
01:02
Introduction
01:41
Preparation of Bacteria
03:49
Preparing Cells for Microscopy
07:16
Visualizing and Analyzing the Cells
09:29
Representative Microscopy Results
10:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们展示了单分子荧光显微镜,对生活的细菌细胞,使功能分子复合物进行检测,跟踪和量化的执行协议。
Tags
Advanced Fluorescence Microscopy
Single Molecular Complexes
In Vivo Visualization
Living Cells
Cellular Mechanisms
Biological Systems
Single-molecule Experimentation
Bulk Ensemble-average Measurements
Stochastic Events
Heterogeneous Nature
Optical Microscopy
Analytical Image Analysis Tools
Monitoring Proteins
Living Bacterial Cell
Molecular Complexes
Functioning Biological Machines
Physiologically Relevant Techniques
Minimally-perturbative
Non-invasive
Fluorescently-tagged Protein
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