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Developmental Biology

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JoVE Journal Developmental Biology
Metoder til at studere Mrp4-holdige Makromolekylær Komplekser i forordningen af ​​fibroblast Migration
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Journal (Developmental Biology)
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Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
Journal (Developmental Biology)
Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
 
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Metoder til at studere Mrp4-holdige Makromolekylær Komplekser i forordningen af ​​fibroblast Migration

Article DOI: 10.3791/53973-v • 10:43 min • May 19th, 2016
May 19th, 2016 •
*1,2, *1, 1,2
1Division of Pulmonary Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center, 2Department of Physiology, University of Tennessee Health Science Center
* These authors contributed equally

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MRP4 regulerer forskellige cykliske nukleotid-afhængig signalering arrangementer, herunder en nylig belyst rolle i celle migration. Vi beskriver en direkte, men flerstrenget tilgang at optrævle de nedstrøms molekylære mål for MRP4 resulterer i identifikation af et unikt MRP4 interactome der spiller centrale roller i finjusteret regulering af fibroblast migration.

Tags

Developmental Biology multiresistens protein 4 (MRP4) Actin cAMP-afhængig protein kinase (PKA) Migration Ingenuity Pathway Analysis (IPA) High-Content Mikroskopi Förster resonans (FRET).
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