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JoVE Journal Developmental Biology
In vitro Colony Analyser til karakterisering Tri-potente progenitorceller isoleret fra voksne murine Pancreas
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In vitro Colony Analyser til karakterisering Tri-potente progenitorceller isoleret fra voksne murine Pancreas

Article DOI: 10.3791/54016-v • 09:31 min • June 10th, 2016
June 10th, 2016 •
*1,2, *1, 1, 1, 1, 1, 1, 3, 1
1Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

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In vitro koloni assays til påvisning selvfornyelse og differentiering af stamceller isoleret fra voksne murine bugspytkirtlen er udformet. I disse assays pancreatiske progenitorer medfører cellekolonier i 3-dimensionalt rum i methylcellulose indeholdende halvfast medium. Protokoller for håndtering enkelte celler og karakterisering af de enkelte kolonier beskrives.

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Developmental Biology pancreas kolonidannende enheder progenitorceller fluorescens-aktiveret cellesortering (FACS) enkelt koloni analyser enkelt celle analyser ekstracellulære matrixproteiner laminin hydrogel methylcellulose halvfast medium hel-mount immunfarvning microfluidic PCR voksne mus
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