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An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
JoVE Journal
Medicine
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JoVE Journal Medicine
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
DOI:

08:11 min

August 14, 2016

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Chapters

  • 00:05Title
  • 00:48Orthotopic Breast Cancer Tumor Establishment
  • 02:17Live Mouse Bioluminescent Tumor Growth Imaging
  • 03:24Surgical Primary Tumor Removal
  • 04:36Lung Metastases Validation
  • 06:13Results: Representative Evaluation of Spontaneous Breast Cancer Metastasis
  • 07:00Conclusion

Summary

Automatic Translation

An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.

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