An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

Name: An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
Uploaded: 2016-08-14T13:00:00+00:00
Duration: 8 min 11 s
Description: Watch this Scientific Journal Video about An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis at JoVE.com

Amy V. Paschall; Kebin Liu

doi:10.3791/54040

Journal

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Medicine

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An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

JoVE Journal
Medicine

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JoVE Journal Medicine

An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

DOI:

10.3791/54040-v

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08:11 min

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August 14, 2016

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Amy V. Paschall^2,3, Kebin Liu^2,3

¹Department of Biochemistry and Molecular Biology, Medical College of Georgia,Augusta University, ²Georgia Cancer Center,Augusta University, ³Charlie Norwood VA Medical Center

Chapters

00:05Title
00:48Orthotopic Breast Cancer Tumor Establishment
02:17Live Mouse Bioluminescent Tumor Growth Imaging
03:24Surgical Primary Tumor Removal
04:36Lung Metastases Validation
06:13Results: Representative Evaluation of Spontaneous Breast Cancer Metastasis
07:00Conclusion

Summary

Automatic Translation

An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.