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Journal / Immunology and Infection / Eksperimentel infektion med
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Immunology and Infection

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JoVE Journal Immunology and Infection
Eksperimentel infektion med
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Eksperimentel infektion med

Article DOI: 10.3791/54554-v • 10:10 min • November 16th, 2016
November 16th, 2016 •
*1, *1, *1, 1, 1,2,3
1Department of Immunology, University of Toronto, 2Toronto General Research Institute, University Health Network, 3Women's College Research Institute
* These authors contributed equally

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Denne protokol beskriver, hvordan man pode C57BL / 6J mus med EGD-stamme af Listeria monocytogenes (L. monocytogenes) og måle interferon-γ (IFN-γ) responser ved naturlig dræber (NK) celler, naturlige dræber T (NKT) celler, og adaptive T-lymfocytter efter infektion. Denne protokol beskrives også hvordan man fører overlevelse hos mus efter infektion med en modificeret LD50 dosis af patogenet.

Tags

Infektion , Bakterier infektion model mus flowcytometri interferon-γ respons
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