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Immunology and Infection
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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

DOI:
10.3791/62832-v

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07:56 min

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July 29, 2021

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, , ,
1Molecular Biophysics Unit,Indian Institute of Science, 2Gatan Inc.

Chapters

  • 00:04Introduction
  • 00:40Data Acquisition with Latitude-S
  • 03:34Fine Alignment
  • 04:20Data Acquisition Procedure
  • 05:36Results: Automated Data Acquisition Using Latitude-S for Single-Particle Cryo-Electron Microscopy
  • 07:31Conclusion

Summary

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Single-particle cryo-electron microscopy demands a suitable software package and user-friendly pipeline for high-throughput automatic data acquisition. Here, we present the application of a fully automated image acquisition software package, Latitude-S, and a practical pipeline for data collection of vitrified biomolecules under low-dose conditions.

Tags

1. Automated Data Acquisition Software2. Single-particle Cryo-electron Microscopy3. Structural Characterization4. Biological Macromolecules5. Pathogen Biology6. Latitude-S7. Technique Manager8. Single-Particle Automated Data Collection9. TEM Condition10. Camera Condition11. Atlas State12. Grid State13. Hole State14. Focus State15. Data State16. Defocus Values17. Microscope Settings18. Imaging Optics19. Illumination Conditions20. Camera Exposure Time

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