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Arbovirus infektioner som Screening-værktøjer for identifikation af Viral hæmmende og vært Antiviral faktorer
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Immunology and Infection
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JoVE Journal Immunology and Infection
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus infektioner som Screening-værktøjer for identifikation af Viral hæmmende og vært Antiviral faktorer

DOI:
10.3791/58244-v

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06:02 min

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September 13, 2018

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, ,
1Department of Microbiology,University of Texas Southwestern Medical Center

Chapters

  • 00:04Title
  • 00:48General Lymantria dispar (LD652) Cell and Virus Culture
  • 01:33General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
  • 02:43Luciferase Assay
  • 04:06Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
  • 05:04Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her, protokoller for at identificere 1) virus-kodet hæmmende, der fremmer arbovirus replikering og 2) eukaryote vært faktorer, der begrænser arbovirus replikation. Disse fluorescens - og luminescens-baserede metoder giver forskere hurtigt få kvantitative udlæsninger af arbovirus replikering i forsimplede assays med lavt signal-støj-forhold.

Tags

Keywords: ArbovirusViral ImmunologyHost FactorsViral Immune EvasionLuminescence AssayFluorescent AssayLepidopteran Insect CellsVSV LuciferaseVaccinia VirusReporter Lysis Buffer

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