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Biochemistry

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JoVE Journal Biochemistry
Online Size-udelukkelse og ionbytningskromatografi på en SAXS Beamline
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Online Size-udelukkelse og ionbytningskromatografi på en SAXS Beamline

Article DOI: 10.3791/54861-v • 11:09 min • January 5th, 2017
January 5th, 2017 •
1, 2,3, 4
1Structural Biology Group, European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences, Keele University, 4Groupe de Microscopie Electronique et Méthodes, Institut de Biologie Structurale

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Bestemmelsen af ​​opløsningen struktur af et protein ved lille vinkel røntgenspredning (SAXS) kræver monodisperse prøver. Her præsenterer vi to muligheder for at sikre minimale forsinkelser mellem prøveforberedelse og dataopsamling: online størrelse-(SEC) og online ionbytningskromatografi (IEC).

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Biokemi Bio-Small Angle X-ray Scattering (BioSAXS) online størrelse-(SEC) online ionbytningskromatografi (IEC) baggrund subtraktion D5 vaccinia
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