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Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
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Biochemistry
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JoVE Journal Biochemistry
Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

DOI:
10.3791/57815-v

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07:09 min

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October 26, 2018

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, , , , , ,
1Faculty of Life and Medical Sciences,Doshisha University, 2The Institute of Genetics and Animal Breeding,Polish Academy of Sciences

Chapters

  • 00:04Title
  • 00:48Preparation of Cells
  • 02:41Preparation of Dextran
  • 03:18Preparation for Laser and Microscopy
  • 03:55Cell Manipulation Using the Laser Trapping System
  • 05:30Construction of a 3-Dimensional Cell Structure
  • 06:10Results: Analysis Stable 3D Cellular Assembly
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate a novel method for constructing a single-cell-based 3-dimensional (3D) assembly without an artificial scaffold.

Tags

Keywords: 3D Cellular AssemblyCell ManipulationHydrophilic PolymerDextranNAMRU Mouse Mammary Gland Epithelial CellsTrypsinDMEMFBSPenicillin-StreptomycinCentrifugationCryopreservationLaserCameraLEDFocus AdjustMoving Stage

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