激光诱导SD大鼠慢性高眼压高压模型

Biology

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Summary

青光眼是世界失明的主要成因之一。高眼压是主要危险因素。激光光凝诱导高眼压症,是建立良好的动物模型之一。本视频演示了如何诱导氩激光光凝大鼠高眼压症。

Cite this Article

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Chiu, K., Chang, R., So, K. Laser-Induced Chronic Ocular Hypertension Model on SD Rats. J. Vis. Exp. (10), e549, doi:10.3791/549 (2007).

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Abstract

青光眼是世界失明的主要成因之一。高眼压是主要危险因素。激光光凝诱导高眼压症,是建立良好的动物模型之一。本视频演示了如何诱导氩激光光凝大鼠高眼压症。

Protocol

检查和准备设备

  1. 麻醉大鼠腹腔注射氯胺酮(80mg/kg)和甲苯噻嗪(8mg/kg)(体积比为2:1)。
  2. 应用激光光凝前局部麻醉剂对大鼠眼睛下降0.5%alcaine。
  3. 大鼠的位置,并用弯钳暴露目标静脉。使用的脚步开始激光光凝。申请回合60点激光角膜缘静脉(鼻地区除外)和15-20激光点,在每个巩膜房水引流静脉周围。

激光光凝

  1. 麻醉大鼠腹腔注射氯胺酮(80mg/kg)和甲苯噻嗪(8mg/kg)(体积比为2:1)。
  2. 应用激光光凝前局部麻醉剂对大鼠眼睛下降0.5%alcaine。
  3. 大鼠的位置,并用弯钳暴露目标静脉。使用的脚步开始激光光凝。申请回合60点激光角膜缘静脉(鼻地区除外)和15-20激光点,在每个巩膜房水引流静脉周围。
  4. 每个激光治疗后,适用于眼科Tobrex软膏对大鼠的眼睛,以防止感染。
  5. 转折的关键反时针指向“0”,关闭激光系统。
  6. 通过旋转开关指向“0”关闭裂隙灯。

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Discussion

两个相隔7天的激光治疗后,我们可以提高50%,与对照眼比较激光光凝眼的眼压。高眼压可维持至少第一次激光治疗后3个月。这种高眼压大鼠模型,研究压力诱导的视网膜神经节细胞丧失,视神经损伤,模仿人​​类青光眼的病理改变的机制提供了宝贵的机会。

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Materials

Name Type Company Catalog Number Comments
ULTIMA® 2000SE Argon Laser Tool Coherent Inc.
Protective goggles Other Coherent Inc.

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References

  1. Chan, H. -C., Chang, R. C. C., Koon-Ching Ip, A., Chiu, K., Yuen, W. -H., Zee, S. -Y., So, K. -F. Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma. Experimental Neurology. 203, 269-273 (2007).
  2. Ji, J. -Z., Elyaman, W., Yip, H. K., Lee, V. W. H., Yick, L. -W., Hugon, J., So, K. -F. CNTF promotes survival of retinal ganglion cells after induction of ocular hypertension in rats: the possible involvement of STAT3 pathway. European Journal of Neuroscience. 19, 265-272 (2004).
  3. Li, R. S., Chen, B. -Y., Tay, D. K., Chan, H. H. L., Pu, M. -L., So, K. -F. Melanopsin-Expressing Retinal Ganglion Cells Are More Injury-Resistant in a Chronic Ocular Hypertension Model. Investigative Ophthalmology & Visual Science. 47, 2951-2958 (2006).
  4. Schori, H., Kipnis, J., Yoles, E., WoldeMussie, E., Ruiz, G., Wheeler, L. A., Schwartz, M. Vaccination for protection of retinal ganglion cells against death from glutamate cytotoxicity and ocular hypertension: Implications for glaucoma. PNAS. 98, 3398-3403 (2001).
  5. WoldeMussie, E., Ruiz, G., Wijono, M., Wheeler, L. A. Neuro-protection of retinal ganglion cells by brimonidine in rats with laser-induced chronic ocular hypertension. Investigative Ophthalmology & Visual Science. 42, 2849-2855 (2001).

Comments

1 Comment

  1. Dear Chiu, What about the size of the laser spot? and for the second laser,how to identify the veins? as  the veins  would be  more difficult to identify  after the first laser . Thanks a lot! Best egards, Huang Chukai

    Reply
    Posted by: Anonymous
    January 13, 2009 - 9:40 AM

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