Journal
/
Biology
/
Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

DOI:
10.3791/62228-v

•

08:33 min

•

February 10, 2021

•

, ,
1Ocular Genomics Institute of Massachusetts Eye and Ear,Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:04Collection of RPE
  • 04:09Isolation of Primary Mouse RPE Cells
  • 06:37Results: Morphological and Functional Validation of RPE Cells
  • 07:38Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol, which was originally reported by Fernandez-Godino et al. in 20161, describes a method to efficiently isolate and culture mouse RPE cells, which form a functional and polarized RPE monolayer within one week on Transwell plates. The procedure takes approximately 3 hours.

Tags

Keywords: Primary Mouse Retinal Pigment Epithelial CellsRPE Cell CultureEye DissectionAge-related Macular DegenerationHyaluronidaseHBSS BufferCornea IncisionOra Serrata

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62228?article_type=v

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
check_url/62228?article_type=v

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
check_url/62228?article_type=v

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
check_url/62228?article_type=v

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
check_url/62228?article_type=v

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
check_url/62228?article_type=v

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium
check_url/62228?article_type=v

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
check_url/62228?article_type=v

Isolation of Primary Mouse Retinal Pigmented Epithelium Cells
check_url/62228?article_type=v

Primary Culture of Porcine Retinal Pigment Epithelial Cells
check_url/62228?article_type=v

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Read Article