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Journal / Bioengineering / 2D和3D矩阵研究线性侵袭体形成和活性
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
2D和3D矩阵研究线性侵袭体形成和活性
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2D和3D矩阵研究线性侵袭体形成和活性

Article DOI: 10.3791/54911-v • 12:26 min • June 2nd, 2017
June 2nd, 2017 •
*1,2, *1, 1,3,4, 2, 2, 1, 1
1INSERM U1053, 2Icahn School of Medicine at Mount Sinai, Tisch Cancer Institute, 3Faculté de Médecine, Casablanca, Université Mohammed 6 des Sciences de la Santé (UM6SS), 4Laboratoire National de Référence
* These authors contributed equally

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该协议描述了如何制备由明胶和胶原蛋白I组成的2D混合基质，以及3D胶原I插塞来研究线性侵袭体。这些方案允许研究线性侵袭体形成，基质降解活性和原代细胞和癌细胞系的侵袭能力。

Tags

生物工程，第124期，线粒体，细胞外基质，降解活性，胶原蛋白I，3D入侵
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