JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
3D 基质中哺乳动物细胞分裂的定量共焦反射显微镜观察
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
3D 基质中哺乳动物细胞分裂的定量共焦反射显微镜观察
DOI:
10.3791/56364-v
•
10:22 min
•
November 29, 2017
•
Lijuan He*
1,2
,
Alexandra Sneider*
1
,
Weitong Chen
,
Michelle Karl
,
Vishnu Prasath
,
Pei-Hsun Wu
2
,
Gunnar Mattson
,
Denis Wirtz
2,4
1
Department of Chemical and Biomolecular Engineering
,
Johns Hopkins University
,
2
Johns Hopkins Physical Sciences – Oncology Center
,
Johns Hopkins University
,
3
Department of Biomedical Engineering
,
Johns Hopkins University
,
4
Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center
,
Johns Hopkins University School of Medicine
Chapters
00:05
Title
00:32
Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
04:00
Synchronization of Cells Stably Expressing H2B-mCherry
06:02
Incorporation of the Synchronized Cells into Collagen I Matrices
07:18
Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
08:46
Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
09:45
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议有效地研究了3D 胶原基质中的哺乳动物细胞分裂, 结合细胞分裂的同步, 用活细胞成像技术监测3D 矩阵中的分裂事件, 时间分辨共聚焦反射显微镜和定量成像分析。
Tags
3D Matrices
Mammalian Cell Division
Confocal Reflection Microscopy
HEK-293 T-cells
Lentiviral Vector
Transfection
MDA-MB-231 Cells
Cell Culture
Cell Matrix Interactions
Cell Biology
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
视频率扫描共聚焦显微镜和内窥镜
皮层组织和动态可视化的微生物,利用全内反射荧光显微镜
Nanotopology细胞粘附在可变角度的全内反射荧光显微镜(VA-TIRFM)
相关显微镜的动力相互作用的三维结构分析
定量的光学显微镜:测量细胞生物物理特性与标准光学显微镜
全球舒张功能通过静脉流的通过参数化舒张形式主义运动学建模为基础的定量分析
2D和3D矩阵研究线性侵袭体形成和活性
单细胞基因组测序的超高通量微流控平台
用微计算机断层扫描法对无节段3D 组织学进行固定染色、全尺寸、毫米度试样的刚性嵌入
一种新的三维打印解光矩阵处理方法
Read Article