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Bioengineering
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标准化和可扩展的测定,以研究iPSC衍生内皮细胞在Vitro的渗透3D血管生成发芽
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
标准化和可扩展的测定,以研究iPSC衍生内皮细胞在Vitro的渗透3D血管生成发芽
DOI:
10.3791/59678-v
•
10:47 min
•
November 06, 2019
•
Vincent van Duinen
2
,
Wendy Stam
,
Viola Borgdorff
,
Arie Reijerkerk
,
Valeria Orlova
,
Paul Vulto
,
Thomas Hankemeier
,
Anton Jan van Zonneveld
1
Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine
,
Leiden University Medical Center
,
2
Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics
,
Leiden University
,
3
Ncardia
,
4
Department of Anatomy and Embryology
,
Leiden University Medical Center
,
5
Mimetas
Chapters
00:04
Title
01:12
Preparation of Device, Gel, and Coating
02:42
Cell Seeding/Microvessel Culture
04:37
Study Angiogenic Sprouting
05:49
Study Anastomosis and Sprout Stabilization
06:50
Fixation, Staining, and Imaging
08:55
Results: Cell Seeding and 3D Sprouting of iPSC-EC
10:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方法将iPSC衍生内皮细胞的培养描述为标准化微流体平台中的40个注入3D微容器。该平台支持在3D中研究梯度驱动的血管生成,包括以可扩展的高通量方式血管生成芽的血管生成和稳定。
Tags
3D Angiogenic Sprouting
IPSC-derived Endothelial Cells
In Vitro Assay
Microfluidic Platform
High-throughput Screening
Angiogenesis
Collagen
Fibronectin
Perfusion
Cardiovascular Disease
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