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Developmental Biology

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JoVE Journal Developmental Biology
Samlokalisering av Cell Lineage Markører og tomat Signal
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
Journal (Developmental Biology)
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
Advancing Lens Biomechanics Research Through a Novel Protocol for Imaging Complex Interdigitations and Protein Staining (Video) | JoVE
Journal (Developmental Biology)
Advancing Lens Biomechanics Research Through a Novel Protocol for Imaging Complex Interdigitations and Protein Staining (Video) | JoVE
 
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Samlokalisering av Cell Lineage Markører og tomat Signal

Article DOI: 10.3791/54982-v • 10:56 min • December 28th, 2016
December 28th, 2016 •
1, 1, 1, 1
1Department of Biomedical Sciences, Texas A&M University College of Dentistry

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Vi utviklet to sett med sporing kombinasjoner i Rosa26 tdtomato (overalt uttrykt i alle celler) / Cre (spesielt uttrykt i chondrocytes) mus: en med 2.3Col1a1-GFP (spesifikke for osteoblaster) og ett med immunfluorescens (spesifikke for beinceller). Dataene viser den direkte transformasjon av kondrocytter inn i beinceller.

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Developmental Biology celle avstamning tracing celletransdifferensiering immunfluorescens chondrocyttransplantasjon osteoblast osteocyte
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