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Journal / Bioengineering / Mammalcelleindkapsling i alginatperler ved hjælp…
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Bioengineering
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JoVE Journal Bioengineering
Mammalcelleindkapsling i alginatperler ved hjælp af et enkelt omrørt skib
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Mammalcelleindkapsling i alginatperler ved hjælp af et enkelt omrørt skib

Article DOI: 10.3791/55280-v • 10:21 min • June 29th, 2017
June 29th, 2017 •
1, 2, 2, 3, 1, 1, 2
1Department of Chemical Engineering, McGill University, 2Michael Smith Laboratories & Department of Chemical and Biological Engineering, University of British Columbia, 3Michael Smith Laboratories & Department of Pharmaceutical Sciences, University of British Columbia

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Denne video og manuskript beskriver en emulsionsbaseret fremgangsmåde til indkapsling af pattedyrsceller i 0,5% til 10% alginatperler, der kan fremstilles i store batcher under anvendelse af en simpel omrørt beholder. De indkapslede celler kan dyrkes in vitro eller transplanteres til cellulære terapiapplikationer.

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Bioengineering udgave 124 bugspytkirtlen øen indkapsling alginat hydrogel emulsion intern gelering immunoisolering vævsteknik differentiering
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