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Mammalcelleindkapsling i alginatperler ved hjælp af et enkelt omrørt skib
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Bioengineering
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JoVE Journal Bioengineering
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Mammalcelleindkapsling i alginatperler ved hjælp af et enkelt omrørt skib

DOI:
10.3791/55280-v

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10:20 min

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June 29, 2017

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, , , , , ,
1Department of Chemical Engineering,McGill University, 2Michael Smith Laboratories & Department of Chemical and Biological Engineering,University of British Columbia, 3Michael Smith Laboratories & Department of Pharmaceutical Sciences,University of British Columbia

Chapters

  • 00:00Title
  • 00:10Introduction
  • 02:49Preparation of Alginate Solution, CaCO3 Suspension and Acidified Oil
  • 04:56Alginate Bead Generation by Emulsion and Internal Gelation
  • 06:45Bead Recovery
  • 08:19Representative Cell Immobilization Results
  • 09:11Conclusion

Summary

Automatic Translation

Automatic Translation

Denne video og manuskript beskriver en emulsionsbaseret fremgangsmåde til indkapsling af pattedyrsceller i 0,5% til 10% alginatperler, der kan fremstilles i store batcher under anvendelse af en simpel omrørt beholder. De indkapslede celler kan dyrkes in vitro eller transplanteres til cellulære terapiapplikationer.

Tags

Cell EncapsulationAlginate BeadsStirred VesselEmulsionInternal GelationCalcium CarbonateAcetic AcidImmunoisolationTransplantationScalabilityRobustnessDroplet SizeMechanical PropertiesCell Survival

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