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Mammalcelleindkapsling i alginatperler ved hjælp af et enkelt omrørt skib
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
DOI:

10:20 min

June 29, 2017

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Chapters

  • 00:00Title
  • 00:10Introduction
  • 02:49Preparation of Alginate Solution, CaCO3 Suspension and Acidified Oil
  • 04:56Alginate Bead Generation by Emulsion and Internal Gelation
  • 06:45Bead Recovery
  • 08:19Representative Cell Immobilization Results
  • 09:11Conclusion

Summary

Automatic Translation

Denne video og manuskript beskriver en emulsionsbaseret fremgangsmåde til indkapsling af pattedyrsceller i 0,5% til 10% alginatperler, der kan fremstilles i store batcher under anvendelse af en simpel omrørt beholder. De indkapslede celler kan dyrkes in vitro eller transplanteres til cellulære terapiapplikationer.

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