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Bioengineering
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使用简单搅拌容器在藻酸盐珠中的哺乳动物细胞包封
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Bioengineering
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JoVE Journal
Bioengineering
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
Please note that all translations are automatically generated.
Click here for the English version.
使用简单搅拌容器在藻酸盐珠中的哺乳动物细胞包封
DOI:
10.3791/55280-v
•
10:20 min
•
June 29, 2017
•
Corinne A. Hoesli
,
Roger L. J. Kiang
,
Kamini Raghuram
,
René G. Pedroza
,
Karen E. Markwick
,
Antonio M. R. Colantuoni
,
James M. Piret
1
Department of Chemical Engineering
,
McGill University
,
2
Michael Smith Laboratories & Department of Chemical and Biological Engineering
,
University of British Columbia
,
3
Michael Smith Laboratories & Department of Pharmaceutical Sciences
,
University of British Columbia
Chapters
00:00
Title
00:10
Introduction
02:49
Preparation of Alginate Solution, CaCO
3
Suspension and Acidified Oil
04:56
Alginate Bead Generation by Emulsion and Internal Gelation
06:45
Bead Recovery
08:19
Representative Cell Immobilization Results
09:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该视频和手稿描述了一种基于乳液的方法,以将哺乳动物细胞包封在0.5%至10%的藻酸盐珠中,其可以使用简单的搅拌容器以大批量生产。包封的细胞可以
在体外培养
或移植用于细胞治疗应用。
Tags
Cell Encapsulation
Alginate Beads
Stirred Vessel
Emulsion
Internal Gelation
Calcium Carbonate
Acetic Acid
Immunoisolation
Transplantation
Scalability
Robustness
Droplet Size
Mechanical Properties
Cell Survival
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