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Chapters
- 00:05Title
- 01:20Generation of Fluorescent Protein Cassettes by PCR
- 03:26Transform Candida Cells with Fluorescent Protein DNA Cassette
- 07:31Results: Generation of GFP and mCherry Fusions to Eno1
- 08:46Conclusion
PCR-mediated gene modification can be used to generate fluorescent protein fusions in Candida species, which facilitates visualization and quantitation of yeast cells and proteins. Herein, we present a strategy for constructing a fluorescent protein fusion (Eno1-FP) in Candida parapsilosis.
