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Journal / Biochemistry / Auflösen von Affinity Purified Protein-Komplexen…
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Biochemistry

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JoVE Journal Biochemistry
Auflösen von Affinity Purified Protein-Komplexen von Blue native PAGE und Protein Correlation Profilieren
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Auflösen von Affinity Purified Protein-Komplexen von Blue native PAGE und Protein Correlation Profilieren

Article DOI: 10.3791/55498-v • 09:35 min • April 1st, 2017
April 1st, 2017 •
1, 1, 1, 1
1Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute

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Hier stellen wir Protokolle für die Affinitätsreinigung von Proteinkomplexen und deren Trennung durch blaue native PAGE, gefolgt von Proteinkorrelationsprofilierungs freie quantitative Massenspektrometrie Label. Diese Methode ist nützlich Interaktom in verschiedene Proteinkomplexe zu lösen.

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Biochemistry Heft 122 Blau native PAGE Proteinkorrelations Profilieren Proteinkomplex Affinitätsreinigung quantitative Massenspektrometrie Interaktom AP-MS Protein-Wechselwirkung
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